superresolution inverted microscope s Search Results


csu w1 sora superresolution spinning disk confocal  (Yokogawa Electric)
99
Yokogawa Electric csu w1 sora superresolution spinning disk confocal
Figure 5 Higher affinity 7G3 CAR-NK cells demonstrate increased clustering and cytotoxicity in a short-term time lapse. (A) Representative high-resolution confocal images of the immunological synapse formed between primary CAR-NK cells (untransduced (UTD) or expressing 7G3WT or 7G3L CARs) and GFP-tagged MOLM13 target cells (green). Phalloidin-555 was used to stain actin (red) and Pacific Blue was used to stain cytotoxic perforin granules (blue). Images were captured with a Leica Spinning Disk Confocal microscope at 60X in oil. (B–E) Representative final images from a 30 min time lapse demonstrating LysoTracker blue-stained NK cells (blue) (B) and DRAQ7-stained dead cells (white) (D) after co-culture with MOLM13 target cells, with quantification of normalized mean gray value for each channel (C, E). MOLM13 target cells without NK cell co-culture (No NK) were included as an additional control. Each dashed line represents an individual donor with the mean value from n=3 donors depicted by the solid curve. (F–I) Mean speed (F), maximum speed (G), total distance (H), and maximum distance (I) traveled by NK cells from each treatment group after co-culture with MOLM13 target cells following a 30 min time lapse, as determined through single cell tracking. Individual dots represent individual NK cells from pooled experiments with n=3 donors. Images were captured with a Nikon Eclipse Ti2-E fully motorized inverted microscope with a Yokogawa <t>CSU-W1</t> <t>SoRa</t> super- resolution spinning disk confocal and 20×/0.45 s Plan Fluor objective. All imaging analysis was performed in Fiji Distribution of ImageJ software47 with use of the TrackMate plugin48 for single cell tracking experiments (C). Detailed statistical data are provided in online supplemental table 2. ****p<0.0001.
Csu W1 Sora Superresolution Spinning Disk Confocal, supplied by Yokogawa Electric, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ix83 inverted confocal microscope  (Olympus)
96
Olympus ix83 inverted confocal microscope
Figure 5 Higher affinity 7G3 CAR-NK cells demonstrate increased clustering and cytotoxicity in a short-term time lapse. (A) Representative high-resolution confocal images of the immunological synapse formed between primary CAR-NK cells (untransduced (UTD) or expressing 7G3WT or 7G3L CARs) and GFP-tagged MOLM13 target cells (green). Phalloidin-555 was used to stain actin (red) and Pacific Blue was used to stain cytotoxic perforin granules (blue). Images were captured with a Leica Spinning Disk Confocal microscope at 60X in oil. (B–E) Representative final images from a 30 min time lapse demonstrating LysoTracker blue-stained NK cells (blue) (B) and DRAQ7-stained dead cells (white) (D) after co-culture with MOLM13 target cells, with quantification of normalized mean gray value for each channel (C, E). MOLM13 target cells without NK cell co-culture (No NK) were included as an additional control. Each dashed line represents an individual donor with the mean value from n=3 donors depicted by the solid curve. (F–I) Mean speed (F), maximum speed (G), total distance (H), and maximum distance (I) traveled by NK cells from each treatment group after co-culture with MOLM13 target cells following a 30 min time lapse, as determined through single cell tracking. Individual dots represent individual NK cells from pooled experiments with n=3 donors. Images were captured with a Nikon Eclipse Ti2-E fully motorized inverted microscope with a Yokogawa <t>CSU-W1</t> <t>SoRa</t> super- resolution spinning disk confocal and 20×/0.45 s Plan Fluor objective. All imaging analysis was performed in Fiji Distribution of ImageJ software47 with use of the TrackMate plugin48 for single cell tracking experiments (C). Detailed statistical data are provided in online supplemental table 2. ****p<0.0001.
Ix83 Inverted Confocal Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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n storm c2þ superresolution system  (Nikon)
97
Nikon n storm c2þ superresolution system
Figure 5 Higher affinity 7G3 CAR-NK cells demonstrate increased clustering and cytotoxicity in a short-term time lapse. (A) Representative high-resolution confocal images of the immunological synapse formed between primary CAR-NK cells (untransduced (UTD) or expressing 7G3WT or 7G3L CARs) and GFP-tagged MOLM13 target cells (green). Phalloidin-555 was used to stain actin (red) and Pacific Blue was used to stain cytotoxic perforin granules (blue). Images were captured with a Leica Spinning Disk Confocal microscope at 60X in oil. (B–E) Representative final images from a 30 min time lapse demonstrating LysoTracker blue-stained NK cells (blue) (B) and DRAQ7-stained dead cells (white) (D) after co-culture with MOLM13 target cells, with quantification of normalized mean gray value for each channel (C, E). MOLM13 target cells without NK cell co-culture (No NK) were included as an additional control. Each dashed line represents an individual donor with the mean value from n=3 donors depicted by the solid curve. (F–I) Mean speed (F), maximum speed (G), total distance (H), and maximum distance (I) traveled by NK cells from each treatment group after co-culture with MOLM13 target cells following a 30 min time lapse, as determined through single cell tracking. Individual dots represent individual NK cells from pooled experiments with n=3 donors. Images were captured with a Nikon Eclipse Ti2-E fully motorized inverted microscope with a Yokogawa <t>CSU-W1</t> <t>SoRa</t> super- resolution spinning disk confocal and 20×/0.45 s Plan Fluor objective. All imaging analysis was performed in Fiji Distribution of ImageJ software47 with use of the TrackMate plugin48 for single cell tracking experiments (C). Detailed statistical data are provided in online supplemental table 2. ****p<0.0001.
N Storm C2þ Superresolution System, supplied by Nikon, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 97 stars, based on 1 article reviews
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lsm 980 airyscan2 inverted confocal laser scanning superresolution microscope  (Carl Zeiss)
90
Carl Zeiss lsm 980 airyscan2 inverted confocal laser scanning superresolution microscope
Figure 5 Higher affinity 7G3 CAR-NK cells demonstrate increased clustering and cytotoxicity in a short-term time lapse. (A) Representative high-resolution confocal images of the immunological synapse formed between primary CAR-NK cells (untransduced (UTD) or expressing 7G3WT or 7G3L CARs) and GFP-tagged MOLM13 target cells (green). Phalloidin-555 was used to stain actin (red) and Pacific Blue was used to stain cytotoxic perforin granules (blue). Images were captured with a Leica Spinning Disk Confocal microscope at 60X in oil. (B–E) Representative final images from a 30 min time lapse demonstrating LysoTracker blue-stained NK cells (blue) (B) and DRAQ7-stained dead cells (white) (D) after co-culture with MOLM13 target cells, with quantification of normalized mean gray value for each channel (C, E). MOLM13 target cells without NK cell co-culture (No NK) were included as an additional control. Each dashed line represents an individual donor with the mean value from n=3 donors depicted by the solid curve. (F–I) Mean speed (F), maximum speed (G), total distance (H), and maximum distance (I) traveled by NK cells from each treatment group after co-culture with MOLM13 target cells following a 30 min time lapse, as determined through single cell tracking. Individual dots represent individual NK cells from pooled experiments with n=3 donors. Images were captured with a Nikon Eclipse Ti2-E fully motorized inverted microscope with a Yokogawa <t>CSU-W1</t> <t>SoRa</t> super- resolution spinning disk confocal and 20×/0.45 s Plan Fluor objective. All imaging analysis was performed in Fiji Distribution of ImageJ software47 with use of the TrackMate plugin48 for single cell tracking experiments (C). Detailed statistical data are provided in online supplemental table 2. ****p<0.0001.
Lsm 980 Airyscan2 Inverted Confocal Laser Scanning Superresolution Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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superresolution inverted microscope s  (Nikon)
99
Nikon superresolution inverted microscope s
Figure 5 Higher affinity 7G3 CAR-NK cells demonstrate increased clustering and cytotoxicity in a short-term time lapse. (A) Representative high-resolution confocal images of the immunological synapse formed between primary CAR-NK cells (untransduced (UTD) or expressing 7G3WT or 7G3L CARs) and GFP-tagged MOLM13 target cells (green). Phalloidin-555 was used to stain actin (red) and Pacific Blue was used to stain cytotoxic perforin granules (blue). Images were captured with a Leica Spinning Disk Confocal microscope at 60X in oil. (B–E) Representative final images from a 30 min time lapse demonstrating LysoTracker blue-stained NK cells (blue) (B) and DRAQ7-stained dead cells (white) (D) after co-culture with MOLM13 target cells, with quantification of normalized mean gray value for each channel (C, E). MOLM13 target cells without NK cell co-culture (No NK) were included as an additional control. Each dashed line represents an individual donor with the mean value from n=3 donors depicted by the solid curve. (F–I) Mean speed (F), maximum speed (G), total distance (H), and maximum distance (I) traveled by NK cells from each treatment group after co-culture with MOLM13 target cells following a 30 min time lapse, as determined through single cell tracking. Individual dots represent individual NK cells from pooled experiments with n=3 donors. Images were captured with a Nikon Eclipse Ti2-E fully motorized inverted microscope with a Yokogawa <t>CSU-W1</t> <t>SoRa</t> super- resolution spinning disk confocal and 20×/0.45 s Plan Fluor objective. All imaging analysis was performed in Fiji Distribution of ImageJ software47 with use of the TrackMate plugin48 for single cell tracking experiments (C). Detailed statistical data are provided in online supplemental table 2. ****p<0.0001.
Superresolution Inverted Microscope S, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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superresolution inverted microscope s - by Bioz Stars, 2026-05
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elyra ps.1 superresolution inverted microscope  (Carl Zeiss)
90
Carl Zeiss elyra ps.1 superresolution inverted microscope
Figure 5 Higher affinity 7G3 CAR-NK cells demonstrate increased clustering and cytotoxicity in a short-term time lapse. (A) Representative high-resolution confocal images of the immunological synapse formed between primary CAR-NK cells (untransduced (UTD) or expressing 7G3WT or 7G3L CARs) and GFP-tagged MOLM13 target cells (green). Phalloidin-555 was used to stain actin (red) and Pacific Blue was used to stain cytotoxic perforin granules (blue). Images were captured with a Leica Spinning Disk Confocal microscope at 60X in oil. (B–E) Representative final images from a 30 min time lapse demonstrating LysoTracker blue-stained NK cells (blue) (B) and DRAQ7-stained dead cells (white) (D) after co-culture with MOLM13 target cells, with quantification of normalized mean gray value for each channel (C, E). MOLM13 target cells without NK cell co-culture (No NK) were included as an additional control. Each dashed line represents an individual donor with the mean value from n=3 donors depicted by the solid curve. (F–I) Mean speed (F), maximum speed (G), total distance (H), and maximum distance (I) traveled by NK cells from each treatment group after co-culture with MOLM13 target cells following a 30 min time lapse, as determined through single cell tracking. Individual dots represent individual NK cells from pooled experiments with n=3 donors. Images were captured with a Nikon Eclipse Ti2-E fully motorized inverted microscope with a Yokogawa <t>CSU-W1</t> <t>SoRa</t> super- resolution spinning disk confocal and 20×/0.45 s Plan Fluor objective. All imaging analysis was performed in Fiji Distribution of ImageJ software47 with use of the TrackMate plugin48 for single cell tracking experiments (C). Detailed statistical data are provided in online supplemental table 2. ****p<0.0001.
Elyra Ps.1 Superresolution Inverted Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/elyra ps.1 superresolution inverted microscope/product/Carl Zeiss
Average 90 stars, based on 1 article reviews
elyra ps.1 superresolution inverted microscope - by Bioz Stars, 2026-05
90/100 stars
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te2000 inverted microscope  (Nikon)
99
Nikon te2000 inverted microscope
Figure 5 Higher affinity 7G3 CAR-NK cells demonstrate increased clustering and cytotoxicity in a short-term time lapse. (A) Representative high-resolution confocal images of the immunological synapse formed between primary CAR-NK cells (untransduced (UTD) or expressing 7G3WT or 7G3L CARs) and GFP-tagged MOLM13 target cells (green). Phalloidin-555 was used to stain actin (red) and Pacific Blue was used to stain cytotoxic perforin granules (blue). Images were captured with a Leica Spinning Disk Confocal microscope at 60X in oil. (B–E) Representative final images from a 30 min time lapse demonstrating LysoTracker blue-stained NK cells (blue) (B) and DRAQ7-stained dead cells (white) (D) after co-culture with MOLM13 target cells, with quantification of normalized mean gray value for each channel (C, E). MOLM13 target cells without NK cell co-culture (No NK) were included as an additional control. Each dashed line represents an individual donor with the mean value from n=3 donors depicted by the solid curve. (F–I) Mean speed (F), maximum speed (G), total distance (H), and maximum distance (I) traveled by NK cells from each treatment group after co-culture with MOLM13 target cells following a 30 min time lapse, as determined through single cell tracking. Individual dots represent individual NK cells from pooled experiments with n=3 donors. Images were captured with a Nikon Eclipse Ti2-E fully motorized inverted microscope with a Yokogawa <t>CSU-W1</t> <t>SoRa</t> super- resolution spinning disk confocal and 20×/0.45 s Plan Fluor objective. All imaging analysis was performed in Fiji Distribution of ImageJ software47 with use of the TrackMate plugin48 for single cell tracking experiments (C). Detailed statistical data are provided in online supplemental table 2. ****p<0.0001.
Te2000 Inverted Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/te2000 inverted microscope/product/Nikon
Average 99 stars, based on 1 article reviews
te2000 inverted microscope - by Bioz Stars, 2026-05
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ix73 inverted optical microscope  (Olympus)
97
Olympus ix73 inverted optical microscope
Figure 5 Higher affinity 7G3 CAR-NK cells demonstrate increased clustering and cytotoxicity in a short-term time lapse. (A) Representative high-resolution confocal images of the immunological synapse formed between primary CAR-NK cells (untransduced (UTD) or expressing 7G3WT or 7G3L CARs) and GFP-tagged MOLM13 target cells (green). Phalloidin-555 was used to stain actin (red) and Pacific Blue was used to stain cytotoxic perforin granules (blue). Images were captured with a Leica Spinning Disk Confocal microscope at 60X in oil. (B–E) Representative final images from a 30 min time lapse demonstrating LysoTracker blue-stained NK cells (blue) (B) and DRAQ7-stained dead cells (white) (D) after co-culture with MOLM13 target cells, with quantification of normalized mean gray value for each channel (C, E). MOLM13 target cells without NK cell co-culture (No NK) were included as an additional control. Each dashed line represents an individual donor with the mean value from n=3 donors depicted by the solid curve. (F–I) Mean speed (F), maximum speed (G), total distance (H), and maximum distance (I) traveled by NK cells from each treatment group after co-culture with MOLM13 target cells following a 30 min time lapse, as determined through single cell tracking. Individual dots represent individual NK cells from pooled experiments with n=3 donors. Images were captured with a Nikon Eclipse Ti2-E fully motorized inverted microscope with a Yokogawa <t>CSU-W1</t> <t>SoRa</t> super- resolution spinning disk confocal and 20×/0.45 s Plan Fluor objective. All imaging analysis was performed in Fiji Distribution of ImageJ software47 with use of the TrackMate plugin48 for single cell tracking experiments (C). Detailed statistical data are provided in online supplemental table 2. ****p<0.0001.
Ix73 Inverted Optical Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ix73 inverted optical microscope/product/Olympus
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eclipse ti2 inverted microscope  (Nikon)
99
Nikon eclipse ti2 inverted microscope
Figure 5 Higher affinity 7G3 CAR-NK cells demonstrate increased clustering and cytotoxicity in a short-term time lapse. (A) Representative high-resolution confocal images of the immunological synapse formed between primary CAR-NK cells (untransduced (UTD) or expressing 7G3WT or 7G3L CARs) and GFP-tagged MOLM13 target cells (green). Phalloidin-555 was used to stain actin (red) and Pacific Blue was used to stain cytotoxic perforin granules (blue). Images were captured with a Leica Spinning Disk Confocal microscope at 60X in oil. (B–E) Representative final images from a 30 min time lapse demonstrating LysoTracker blue-stained NK cells (blue) (B) and DRAQ7-stained dead cells (white) (D) after co-culture with MOLM13 target cells, with quantification of normalized mean gray value for each channel (C, E). MOLM13 target cells without NK cell co-culture (No NK) were included as an additional control. Each dashed line represents an individual donor with the mean value from n=3 donors depicted by the solid curve. (F–I) Mean speed (F), maximum speed (G), total distance (H), and maximum distance (I) traveled by NK cells from each treatment group after co-culture with MOLM13 target cells following a 30 min time lapse, as determined through single cell tracking. Individual dots represent individual NK cells from pooled experiments with n=3 donors. Images were captured with a Nikon Eclipse Ti2-E fully motorized inverted microscope with a Yokogawa <t>CSU-W1</t> <t>SoRa</t> super- resolution spinning disk confocal and 20×/0.45 s Plan Fluor objective. All imaging analysis was performed in Fiji Distribution of ImageJ software47 with use of the TrackMate plugin48 for single cell tracking experiments (C). Detailed statistical data are provided in online supplemental table 2. ****p<0.0001.
Eclipse Ti2 Inverted Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/eclipse ti2 inverted microscope/product/Nikon
Average 99 stars, based on 1 article reviews
eclipse ti2 inverted microscope - by Bioz Stars, 2026-05
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ti eclipse inverted microscope  (Nikon)
96
Nikon ti eclipse inverted microscope
Figure 5 Higher affinity 7G3 CAR-NK cells demonstrate increased clustering and cytotoxicity in a short-term time lapse. (A) Representative high-resolution confocal images of the immunological synapse formed between primary CAR-NK cells (untransduced (UTD) or expressing 7G3WT or 7G3L CARs) and GFP-tagged MOLM13 target cells (green). Phalloidin-555 was used to stain actin (red) and Pacific Blue was used to stain cytotoxic perforin granules (blue). Images were captured with a Leica Spinning Disk Confocal microscope at 60X in oil. (B–E) Representative final images from a 30 min time lapse demonstrating LysoTracker blue-stained NK cells (blue) (B) and DRAQ7-stained dead cells (white) (D) after co-culture with MOLM13 target cells, with quantification of normalized mean gray value for each channel (C, E). MOLM13 target cells without NK cell co-culture (No NK) were included as an additional control. Each dashed line represents an individual donor with the mean value from n=3 donors depicted by the solid curve. (F–I) Mean speed (F), maximum speed (G), total distance (H), and maximum distance (I) traveled by NK cells from each treatment group after co-culture with MOLM13 target cells following a 30 min time lapse, as determined through single cell tracking. Individual dots represent individual NK cells from pooled experiments with n=3 donors. Images were captured with a Nikon Eclipse Ti2-E fully motorized inverted microscope with a Yokogawa <t>CSU-W1</t> <t>SoRa</t> super- resolution spinning disk confocal and 20×/0.45 s Plan Fluor objective. All imaging analysis was performed in Fiji Distribution of ImageJ software47 with use of the TrackMate plugin48 for single cell tracking experiments (C). Detailed statistical data are provided in online supplemental table 2. ****p<0.0001.
Ti Eclipse Inverted Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ix73 inverted optical microscope  (Evident Corporation)
99
Evident Corporation ix73 inverted optical microscope
Figure 5 Higher affinity 7G3 CAR-NK cells demonstrate increased clustering and cytotoxicity in a short-term time lapse. (A) Representative high-resolution confocal images of the immunological synapse formed between primary CAR-NK cells (untransduced (UTD) or expressing 7G3WT or 7G3L CARs) and GFP-tagged MOLM13 target cells (green). Phalloidin-555 was used to stain actin (red) and Pacific Blue was used to stain cytotoxic perforin granules (blue). Images were captured with a Leica Spinning Disk Confocal microscope at 60X in oil. (B–E) Representative final images from a 30 min time lapse demonstrating LysoTracker blue-stained NK cells (blue) (B) and DRAQ7-stained dead cells (white) (D) after co-culture with MOLM13 target cells, with quantification of normalized mean gray value for each channel (C, E). MOLM13 target cells without NK cell co-culture (No NK) were included as an additional control. Each dashed line represents an individual donor with the mean value from n=3 donors depicted by the solid curve. (F–I) Mean speed (F), maximum speed (G), total distance (H), and maximum distance (I) traveled by NK cells from each treatment group after co-culture with MOLM13 target cells following a 30 min time lapse, as determined through single cell tracking. Individual dots represent individual NK cells from pooled experiments with n=3 donors. Images were captured with a Nikon Eclipse Ti2-E fully motorized inverted microscope with a Yokogawa <t>CSU-W1</t> <t>SoRa</t> super- resolution spinning disk confocal and 20×/0.45 s Plan Fluor objective. All imaging analysis was performed in Fiji Distribution of ImageJ software47 with use of the TrackMate plugin48 for single cell tracking experiments (C). Detailed statistical data are provided in online supplemental table 2. ****p<0.0001.
Ix73 Inverted Optical Microscope, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ix73 inverted optical microscope/product/Evident Corporation
Average 99 stars, based on 1 article reviews
ix73 inverted optical microscope - by Bioz Stars, 2026-05
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Image Search Results


Figure 5 Higher affinity 7G3 CAR-NK cells demonstrate increased clustering and cytotoxicity in a short-term time lapse. (A) Representative high-resolution confocal images of the immunological synapse formed between primary CAR-NK cells (untransduced (UTD) or expressing 7G3WT or 7G3L CARs) and GFP-tagged MOLM13 target cells (green). Phalloidin-555 was used to stain actin (red) and Pacific Blue was used to stain cytotoxic perforin granules (blue). Images were captured with a Leica Spinning Disk Confocal microscope at 60X in oil. (B–E) Representative final images from a 30 min time lapse demonstrating LysoTracker blue-stained NK cells (blue) (B) and DRAQ7-stained dead cells (white) (D) after co-culture with MOLM13 target cells, with quantification of normalized mean gray value for each channel (C, E). MOLM13 target cells without NK cell co-culture (No NK) were included as an additional control. Each dashed line represents an individual donor with the mean value from n=3 donors depicted by the solid curve. (F–I) Mean speed (F), maximum speed (G), total distance (H), and maximum distance (I) traveled by NK cells from each treatment group after co-culture with MOLM13 target cells following a 30 min time lapse, as determined through single cell tracking. Individual dots represent individual NK cells from pooled experiments with n=3 donors. Images were captured with a Nikon Eclipse Ti2-E fully motorized inverted microscope with a Yokogawa CSU-W1 SoRa super- resolution spinning disk confocal and 20×/0.45 s Plan Fluor objective. All imaging analysis was performed in Fiji Distribution of ImageJ software47 with use of the TrackMate plugin48 for single cell tracking experiments (C). Detailed statistical data are provided in online supplemental table 2. ****p<0.0001.

Journal: Journal for immunotherapy of cancer

Article Title: Single-chain variable fragment affinity tuning can optimize anti-AML CAR-NK cell functionality.

doi: 10.1136/jitc-2024-010763

Figure Lengend Snippet: Figure 5 Higher affinity 7G3 CAR-NK cells demonstrate increased clustering and cytotoxicity in a short-term time lapse. (A) Representative high-resolution confocal images of the immunological synapse formed between primary CAR-NK cells (untransduced (UTD) or expressing 7G3WT or 7G3L CARs) and GFP-tagged MOLM13 target cells (green). Phalloidin-555 was used to stain actin (red) and Pacific Blue was used to stain cytotoxic perforin granules (blue). Images were captured with a Leica Spinning Disk Confocal microscope at 60X in oil. (B–E) Representative final images from a 30 min time lapse demonstrating LysoTracker blue-stained NK cells (blue) (B) and DRAQ7-stained dead cells (white) (D) after co-culture with MOLM13 target cells, with quantification of normalized mean gray value for each channel (C, E). MOLM13 target cells without NK cell co-culture (No NK) were included as an additional control. Each dashed line represents an individual donor with the mean value from n=3 donors depicted by the solid curve. (F–I) Mean speed (F), maximum speed (G), total distance (H), and maximum distance (I) traveled by NK cells from each treatment group after co-culture with MOLM13 target cells following a 30 min time lapse, as determined through single cell tracking. Individual dots represent individual NK cells from pooled experiments with n=3 donors. Images were captured with a Nikon Eclipse Ti2-E fully motorized inverted microscope with a Yokogawa CSU-W1 SoRa super- resolution spinning disk confocal and 20×/0.45 s Plan Fluor objective. All imaging analysis was performed in Fiji Distribution of ImageJ software47 with use of the TrackMate plugin48 for single cell tracking experiments (C). Detailed statistical data are provided in online supplemental table 2. ****p<0.0001.

Article Snippet: Images were captured with a Nikon Eclipse Ti2- E fully motorized inverted microscope with a Yokogawa CSU- W1 SoRa superresolution spinning disk confocal and 20×/0.45 s Plan Fluor objective.

Techniques: Expressing, Staining, Microscopy, Co-Culture Assay, Control, Single Cell Tracking, Inverted Microscopy, Imaging